Molecular Therapy
Volume 2, Issue 2, August 2000, Pages 140-146
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Article
Efficient Nonviral Cutaneous Transfection

https://doi.org/10.1006/mthe.2000.0107Get rights and content
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Abstract

Preclinical in vivo rodent, porcine, and primate experiments aimed at enhancing nonviral transgene delivery to skin have been performed. These investigations have identified a compound (aurintricarboxylic acid or ATA) that enhances transfection activity of “naked” plasmid and pulsed electrical fields (electroporation or EP) that synergistically boosts transgene expression to an average of 115-fold more than that observed with free DNA (P < 0.00009). When plasmid is intradermally injected with or without ATA, the transfected cells are typically restricted to the epidermis. However, when electroporation is added after the same injection, larger numbers of adipocytes and fibroblasts and numerous dendritic-like cells within the dermis and subdermal tissues are transfected. This advance creates new opportunities for cutaneous gene therapy and nucleic acid vaccine development.

Keywords

electroporation
transfection
skin
DNA
naked
plasmid
ATA
dermal
aurintricarboxylic
nuclease inhibitor
electropermeabilization
subcutaneous
swine
macaque
rodent

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The views of the authors do not purport to be those of the United States government or the Department of Defense.